Parenchymatous organ injuries affected highly vascularized organs such as liver, spleen or kidneys, become a major challenge in surgical practice, mainly because of tendency to life-threatening bleeding, complex tissue architecture and susceptibility to infections. Using biological polymers as hemostatic agents is important both for stopping bleeding and improving tissue regeneration. Medicines based on such substances are in high demand, they integrated into military medicine, civilian emergency care, surgical interventions and treatment of chronic wounds. Cross-linked chitosan-collagen systems have become a breakthrough solution for hemostasis in parenchymatous organ injuries. In this article we will demonstrate the latest innovations based on interaction of biological polymers such as chitosan and collagen. We will uncover modern processes of their production, purification and transformation, present a full analysis of pharmacodynamic and pharmacokinetic differences between hydrogel and spongy collagen-chitosan systems, compare the preclinical data and clinical studies to understand the use of a specific medical form.